National CryoEM Facility Acknowledged User Publications
The National Cryo-Electron Microscopy Facility (NCEF), which began operation in May 2017, provides cancer researchers access to the latest cryo-EM technology for high resolution imaging. Publications that report macromolecular structures obtained using data collected at NCEF are listed below.
Structural basis of Cas9 DNA interrogation with a 5' truncated sgRNA
Kiernan KA, Kwon J, Merrill BJ, Simonović M
Nucleic Acids Res. 2024
PubMed ID:39657754
Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR function
Kordon SP, Cechova K, Bandekar SJ, Leon K, Dutka P, Siffer G, Kossiakoff AA, Vafabakhsh R, Araç D
Nat Commun. 2024
PubMed ID: 39627215
Structural diversity and clustering of bacterial flagellar outer domains
Fields JL, Zhang H, Bellis NF, Petersen HA, Halder SK, Rich-New ST, Krupovic M, Wu H, Wang F
Nat Commun. 2024
PubMed ID: 39489766
Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor
Lokareddy RK, Hou CD, Forti F, Iglesias SM, Li F, Pavlenok M, Horner DS, Niederweis M, Briani F, Cingolani G
Nat Commun. 2024
PubMed ID: 39353939
Transcription start site scanning requires the fungi-specific hydrophobic loop of Tfb3
Yang C, Basnet P, Sharmin S, Shen H, Kaplan CD, Murakami K
Nucleic Acids Res. 2024
PubMed ID: 39287137
Cell-Free Nonequilibrium Assembly for Hierarchical Protein/Peptide Nanopillars
Guo J, Zia A, Qiu Q, Norton M, Qiu K, Usuba J, Liu Z, Yi M, Rich-New ST, Hagan M, Fraden S, Han GD, Diao J, Wang F, Xu B
J Am Chem Soc. 2024
PubMed ID: 39255453
Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C
Nucleic Acids Res. 2024
PubMed ID: 39149911
Single acetylation-mimetic mutation in TDP-43 Nuclear Localization Signal disrupts importin α1/β signaling
Ko YH, Lokareddy RK, Doll SG, Yeggoni DP, Girdhar A, Mawn I, Klim JR, Rizvi NF, Meyers R, Gillilan RE, Guo L, Cingolani G
J Mol Biol. 2024
PubMed ID: 39181183
Structural basis for substrate binding and selection by human mitochondrial RNA polymerase
Herbine K, Nayak AR, Temiakov D
Nat Commun. 2024
PubMed ID: 39164235
DdmDE eliminates plasmid invasion by DNA-guided DNA targeting
Yang XY, Shen Z, Wang C, Nakanishi K, Fu TM
Cell. 2024
PubMed ID: 39173632
Multi-layered heterochromatin interaction as a switch for DIM2-mediated DNA methylation
Shao Z, Lu J, Khudaverdyan N, Song J
Nat Commun. 2024
PubMed ID: 39122718
Structural dynamics at cytosolic interprotomer interfaces control gating of a mammalian TRPM5 channel
Karuppan S, Schrag LG, Pastrano CM, Jara-Oseguera A, Zubcevic L
Proc Natl Acad Sci U S A. 2024
PubMed ID:38923985
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor
Nayak AR, Rangubpit W, Will AH, Hu Y, Castro-Hartmann P, Lobo JJ, Dryden K, Lamb GD, Sompornpisut P, Samsó M
Nat Commun. 2024
PubMed ID: 38750013
TRPV3 activation by different agonists accompanied by lipid dissociation from the vanilloid site
Kirill D. Nadezhdin, Arthur Neuberger, Lena S. Khosrof, Irina A. Talyzina, Jeffrey Khau, Maria V. Yelshanskaya, Alexander I. Sobolevsky
Sci Adv. 2024
PubMed ID: 38691614
Molecular basis of Gabija anti-phage supramolecular assemblies
Yang XY, Shen Z, Xie J, Greenwald J, Marathe I, Lin Q, Xie WJ, Wysocki VH, Fu TM
Nat Struct Mol Biol. 2024
PubMed ID: 38627580
Antiviral drug recognition and elevator-type transport motions of CNT3
Wright NJ, Zhang F, Suo Y, Kong L, Yin Y, Fedor JG, Sharma K, Borgnia MJ, Im W, Lee SY
Nat Chem Biol. 2024
PubMed ID: 38418906
Structure of Escherichia coli exonuclease VII
Liu C, Hauk G, Yan Q, Berger JM
Proc Natl Acad Sci U S A. 2024
PubMed ID: 38271335
Structural basis for nuclear import of hepatitis B virus (HBV) nucleocapsid core
Yang R, Ko YH, Li F, Lokareddy RK, Hou CD, Kim C, Klein S, Antolínez S, Marín JF, Pérez-Segura C, Jarrold MF, Zlotnick A, Hadden-Perilla JA, Cingolani G
Sci Adv. 2024
PubMed ID: 38198557
A conformational selection mechanism of flavivirus NS5 for species-specific STAT2 inhibition
Biswal M, Yao W, Lu J, Chen J, Morrison J, Hai R, Song J
Commun Biol. 2024
PubMed ID: 38195857
PtuA and PtuB assemble into an inflammasome-like oligomer for anti-phage defense
Li Y, Shen Z, Zhang M, Yang XY, Cleary SP, Xie J, Marathe IA, Kostelic M, Greenwald J, Rish AD, Wysocki VH, Chen C, Chen Q, Fu TM, Yu Y
Nat Struct Mol Biol. 2024
PubMed ID: 38177683
Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus
Hou CD, Li F, Iglesias S, Cingolani G
Methods Mol Biol. 2024
PubMed ID: 37966602
Structural basis for DNA proofreading
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun. 2023
PubMed ID: 38151585
Mechanism of histone H2B monoubiquitination by Bre1
Zhao F, Hicks CW, Wolberger C
Nat Struct Mol Bio. 2023
PubMed ID: 37872231
Hierarchical Assembly of Intrinsically Disordered Short Peptides
Guo J, Rich-New ST, Liu C, Huang Y, Tan W, He H, Yi M, Zhang X, Egelman EH, Wang F, Xu B
Chem. 2023
PubMed ID: 38094164
Structural basis for the allosteric regulation and dynamic assembly of DNMT3B
Lu J, Fang J, Zhu H, Liang KL, Khudaverdyan N, Song J
Nucleic Acids Res. 2023
PubMed ID: 37941146
Structural basis of the American mink ACE2 binding by Y453F trimeric spike glycoproteins of SARS-CoV-2
Ahn H, Calderon BM, Fan X, Gao Y, Horgan NL, Jiang N, Blohm DS, Hossain J, Rayyan NWK, Osman SH, Lin X, Currier M, Steel J, Wentworth DE, Zhou B, Liang B
J Med Virol. 2023
PubMed ID: 37842796
CryoEM structure of a therapeutic antibody (favezelimab) bound to human LAG3 determined using a bivalent Fab as fiducial marker
Mishra AK, Shahid S, Karade SS, Agnihotri P, Kolesnikov A, Hasan SS, Mariuzza RA
Structure. 2023
PubMed ID: 37619561
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1
Abini-Agbomson S, Gretarsson K, Shih RM, Hsieh L, Lou T, De Ioannes P, Vasilyev N, Lee R, Wang M, Simon MD, Armache JP, Nudler E, Narlikar G, Liu S, Lu C, Armache KJ
Mol Cell. 2023
PubMed ID: 37595555
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179
Patil DN, Pantalone S, Cao Y, Laboute T, Novick SJ, Singh S, Savino S, Faravelli S, Magnani F, Griffin PR, Singh AK, Forneris F, Martemyanov KA
Sci Signal. 2023
PubMed ID: 37490546
The evolution of archaeal flagellar filaments
Kreutzberger MAB, Cvirkaite-Krupovic V, Liu Y, Baquero DP, Liu J, Sonani RR, Calladine CR, Wang F, Krupovic M, Egelman EH
Proc Natl Acad Sci U S A. 2023
PubMed ID: 37399404
High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217
Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, Cingolani G
Nat Commun. 2023
PubMed ID: 37422479
Chromogranin B (CHGB) is dimorphic and responsible for dominant anion channels delivered to cell surface via regulated secretion
Yadav GP, Wang H, Ouwendijk J, Cross S, Wang Q, Qin F, Verkade P, Zhu MX, Jiang QX
Front Mol Neurosci. 2023
PubMed ID: 37435575
Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer
Rish AD, Shen Z, Chen Z, Zhang N, Zheng Q, Fu TM
Nat Commun. 2023
PubMed ID: 37322069
Cell spheroid creation by transcytotic intercellular gelation
Guo J, Wang F, Huang Y, He H, Tan W, Yi M, Egelman EH, Xu B
Nat Nanotechnol. 2023
PubMed ID: 37217766
Structural mechanisms of TRPM7 activation and inhibition
Nadezhdin KD, Correia L, Narangoda C, Patel DS, Neuberger A, Gudermann T, Kurnikova MG, Chubanov V, Sobolevsky AI
Nat Commun. 2023
PubMed ID: 37156753
HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation
Talledge N, Yang H, Shi K, Coray R, Yu G, Arndt WG, Meng S, Baxter GC, Mendonça LM, Castaño-Díez D, Aihara H, Mansky LM, Zhang W
J Mol Biol. 2023
PubMed ID: 37150290
Human TRPV1 structure and inhibition by the analgesic SB-366791
Neuberger A, Oda M, Nikolaev YA, Nadezhdin KD, Gracheva EO, Bagriantsev SN, Sobolevsky AI
Nat Commun. 2023
PubMed ID: 37117175
The structural basis of tRNA recognition by arginyl-tRNA-protein transferase
Abeywansha T, Huang W, Ye X, Nawrocki A, Lan X, Jankowsky E, Taylor DJ, Zhang Y
Nat Commun. 2023
PubMed ID: 37076488
Structures of human primosome elongation complexes
He Q, Baranovskiy AG, Morstadt LM, Lisova AE, Babayeva ND, Lusk BL, Lim CJ, Tahirov TH
Nat Struct Mol Biol. 2023
PubMed ID: 37069376
SARS-CoV-2 polyprotein substrate regulates the stepwise Mpro cleavage reaction
Narwal M, Armache JP, Edwards TJ, Murakami KS
J Biol Chem. 2023
PubMed ID: 37044215
Cryo-EM structure of the human Sirtuin 6-nucleosome complex
Chio US, Rechiche O, Bryll AR, Zhu J, Leith EM, Feldman JL, Peterson CL, Tan S, Armache JP
Sci Adv. 2023
PubMed ID: 37058572
Single particle cryo-EM analysis of Rickettsia conorii Sca2 reveals a formin-like core
Carman PJ, Rebowski G, Dominguez R, Alqassim SS
J Struct Biol. 2023
PubMed ID: 37028467
Small molecule inhibitors of 15-PGDH exploit a physiologic induced-fit closing system
Huang W, Li H, Kiselar J, Fink SP, Regmi S, Day A, Yuan Y, Chance M, Ready JM, Markowitz SD, Taylor DJ
Nat Commun. 2023
PubMed ID: 36774348
Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein
Kuhn CC, Basnet N, Bodakuntla S, Alvarez-Brecht P, Nichols S, Martinez-Sanchez A, Agostini L, Soh YM, Takagi J, Biertümpfel C, Mizuno N
Nat Commun. 2023
PubMed ID: 36739444
Structural basis of regulated m7G tRNA modification by METTL1-WDR4
Li J, Wang L, Hahn Q, Nowak RP, Viennet T, Orellana EA, Roy Burman SS, Yue H, Hunkeler M, Fontana P, Wu H, Arthanari H, Fischer ES, Gregory RI
Nature. 2023
PubMed ID: 36599985
High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine
Li F, Hou CD, Yang R, Whitehead R 3rd, Teschke CM, Cingolani G
Sci Adv. 2022
PubMed ID: 36475795
A potent and broad neutralization of SARS-CoV-2 variants of concern by DARPins
Chonira V, Kwon YD, Gorman J, Case JB, Ku Z, Simeon R, Casner RG, Harris DR, Olia AS, Stephens T, Shapiro L, Bender MF, Boyd H, Teng IT, Tsybovsky Y, Krammer F, Zhang N, Diamond MS, Kwong PD, An Z, Chen Z
Nat Chem Biol. 2022
PubMed ID: 36411391
Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP2
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science. 2022
PubMed ID: 36227998
Potent monoclonal antibodies neutralize Omicron sublineages and other SARS-CoV-2 variants
Chen Z, Zhang P, Matsuoka Y, Tsybovsky Y, West K, Santos C, Boyd LF, Nguyen H, Pomerenke A, Stephens T, Olia AS, Zhang B, De Giorgi V, Holbrook MR, Gross R, Postnikova E, Garza NL, Johnson RF, Margulies DH, Kwong PD, Alter HJ, Buchholz UJ, Lusso P, Farci P
Cell Rep. 2022
PubMed ID: 36302375
Structures of the human CST-Polα-primase complex bound to telomere templates
He Q, Lin X, Chavez BL, Agrawal S, Lusk BL, Lim CJ
Nature. 2022
PubMed ID: 35830881
Cryo-EM structure-based selection of computed ligand poses enables design of MTA-synergic PRMT5 inhibitors of better potency
Zhou W, Yadav GP, Yang X, Qin F, Li C, Jiang QX
Commun Biol. 2022
PubMed ID: 36192627
Structural Basis of Peptide-Based Antimicrobial Inhibition of a Resistance-Nodulation-Cell Division Multidrug Efflux Pump
Lyu M, Ayala JC, Chirakos I, Su CC, Shafer WM, Yu EW
Microbiol Spectr. 2022
PubMed ID: 36121287
Molecular basis for polysaccharide recognition and modulated ATP hydrolysis by the O antigen ABC transporter
Spellmon N, Muszyński A, Górniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun. 2022
PubMed ID: 36064941
Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P
Zhu J, Huang W, Zhao J, Huynh L, Taylor DJ, Harris ME
Nat Commun. 2022
PubMed ID: 36045135
Cryo-EM Structure and Functional Studies of EBNA1 Binding to the Family of Repeats and Dyad Symmetry Elements of Epstein-Barr Virus oriP
Mei Y, Messick TE, Dheekollu J, Kim HJ, Molugu S, Muñoz LJC, Moiskeenkova-Bell V, Murakami K, Lieberman PM
J Virol. 2022
PubMed ID: 36037477
Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs
Kumar A, Kindig K, Rao S, Zaki AM, Basak S, Sansom MSP, Biggin PC, Chakrapani S
Nat Commun. 2022
PubMed ID: 35982060
Autoantibody mimicry of hormone action at the thyrotropin receptor
Faust B, Billesbølle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature. 2022
PubMed ID: 35940205
Structural analysis of the basal state of the Artemis:DNA-PKcs complex
Watanabe G, Lieber MR, Williams DR
Nucleic Acids Res. 2022
PubMed ID: 35801871
Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators
Selvakumar P, Fernández-Mariño AI, Khanra N, He C, Paquette AJ, Wang B, Huang R, Smider VV, Rice WJ, Swartz KJ, Meyerson JR
Nat Commun. 2022
PubMed: 35788586
Optimization of Benzoxazinorifamycins to Improve Mycobacterium tuberculosis RNA Polymerase Inhibition and Treatment of Tuberculosis
Rajeswaran W, Ashkar SR, Lee PH, Yeomans L, Shin Y, Franzblau SG, Murakami KS, Showalter HD, Garcia GA
ACS Infect Dis. 2022
PubMed ID: 35772744
Structural mechanism of TRPV3 channel inhibition by the anesthetic dyclonine
Neuberger A, Nadezhdin KD, Sobolevsky AI
Nat Commun. 2022
PubMed ID: 35589741
Phenol-soluble modulins PSMα3 and PSMβ2 form nanotubes that are cross-α amyloids
Kreutzberger MAB, Wang S, Beltran LC, Tuachi A, Zuo X, Egelman EH, Conticello VP
Proc Natl Acad Sci USA. 2022
PubMed ID: 35533283
Structure, substrate recognition and initiation of hyaluronan synthase
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature. 2022
PubMed ID: 35355017
Structural basis for potent antibody neutralization of SARS-CoV-2 variants including B.1.1.529
Zhou T, Wang L, Misasi J, Pegu A, Zhang Y, Harris DR, Olia AS, Talana CA, Yang ES, Chen M, Choe M, Shi W, Teng IT, Creanga A, Jenkins C, Leung K, Liu T, Stancofski ED, Stephens T, Zhang B, Tsybovsky Y, Graham BS, Mascola JR, Sullivan NJ, Kwong PD
Science. 2022
PubMed ID: 35324257
Cryo-EM structure of a kinetically trapped dodecameric portal protein from the Pseudomonas-phage PaP3
David Hou CF, Swanson NA, Li F, Yang R, Lokareddy RK, Cingolani G
J Mol Biol. 2022
PubMed ID: 35278476
Ca2+-inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM
Nayak AR, Samsó M
Elife. 2022
PubMed ID: 35257661
Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state
Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP
Nat Struct Mol Bio 2022
PubMed ID: 35173352
Molecular mechanism of Arp2/3 complex inhibition by Arpin
Fregoso FE, van Eeuwen T, Simanov G, Rebowski G, Boczkowska M, Zimmet A, Gautreau AM, Dominguez R
Nat Commun. 2022
PubMed ID: 35110533
Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II
Yang C, Fujiwara R, Kim HJ, Basnet P, Zhu Y, Gorbea Colón JJ, Steimle S, Garcia BA, Kaplan CD, Murakami K
Mol Cell. 2022
PubMed ID: 35051353
Structure of putative tumor suppressor ALDH1L1
Tsybovsky Y, Sereda V, Golczak M, Krupenko NI, Krupenko SA
Commun Biol. 2022
PubMed ID: 35013550
Regulation of MLL1 Methyltransferase Activity in Two Distinct Nucleosome Binding Modes
Ayoub A, Park SH, Lee YT, Cho US, Dou Y
Biochemistry. 2021
PubMed ID: 34928138
A molecular mechanism for the generation of ligand-dependent differential outputs by the epidermal growth factor receptor
Huang Y, Ognjenovic J, Karandur D, Miller K, Merk A, Subramaniam S, Kuriyan J
Elife. 2021
PubMed ID: 34846302
Structural insights into Ubr1-mediated N-degron polyubiquitination
Pan M, Zheng Q, Wang T, Liang L, Mao J, Zuo C, Ding R, Ai H, Xie Y, Si D, Yu Y, Liu L, Zhao M
Nature. 2021
PubMed ID: 34789879
Expression and purification of phage T7 ejection proteins for cryo-EM analysis
Swanson NA, Lokareddy RK, Li F, Hou CF, Pavlenok M, Niederweis M, Cingolani G
STAR Protoc. 2021
PubMed ID: 34825220
Cryo-EM structure of human GPR158 receptor coupled to the RGS7-Gβ5 signaling complex
Patil DN, Singh S, Laboute T, Strutzenberg TS, Qiu X, Wu D, Novick SJ, Robinson CV, Griffin PR, Hunt JF, Izard T, Singh AK, Martemyanov KA
Science. 2021
PubMed ID: 34793198
Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli
Qayyum MZ, Molodtsov V, Renda A, Murakami KS
J Biol Chem. 2021
PubMed ID: 34774797
Structural mechanisms of TRPV6 inhibition by ruthenium red and econazole
Neuberger A, Nadezhdin KD, Sobolevsky AI
Nat Commun. 2021
PubMed ID: 34725357
Prestin's conformational cycle underlies outer hair cell electromotility
Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E
Nature. 2021
PubMed ID: 34695838
Expression and characterization of SARS-CoV-2 spike proteins
Schaub JM, Chou CW, Kuo HC, Javanmardi K, Hsieh CL, Goldsmith J, DiVenere AM, Le KC, Wrapp D, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Wang N, Lavinder JJ, Ippolito GC, Maynard JA, McLellan JS, Finkelstein IJ
Nat Protoc. 2021
PubMed ID: 34611365
SARS-CoV-2 S2P spike ages through distinct states with altered immunogenicity
Olia AS, Tsybovsky Y, Chen SJ, Liu C, Nazzari AF, Ou L, Wang L, Kong WP, Leung K, Liu T, Stephens T, Teng IT, Wang S, Yang ES, Zhang B, Zhang Y, Zhou T, Mascola JR, Kwong PD
J Biol Chem. 2021
PubMed ID: 34461095
Structures of the mycobacterial membrane protein MmpL3 reveal its mechanism of lipid transport
Su CC, Klenotic PA, Cui M, Lyu M, Morgan CE, Yu EW
Plos Biol. 2021
PubMed ID: 34383749
Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopes
Sun D, Sang Z, Kim YJ, Xiang Y, Cohen T, Belford AK, Huet A, Conway JF, Sun J, Taylor DJ, Schneidman-Duhovny D, Zhang C, Huang W, Shi Y
Nat Commun. 2021
PubMed ID: 34344900
Mechanistic insight into substrate processing and allosteric inhibition of human p97
Pan M, Yu Y, Ai H, Zheng Q, Xie Y, Liu L, Zhao M
Nat Struct Mol Biol. 2021
PubMed ID: 34262183
Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel
Nadezhdin KD, Neuberger A, Trofimov YA, Krylov NA, Sinica V, Kupko N, Vlachova V, Zakharian E, Efremov RG, Sobolevsky AI
Nat Struct Mol Biol. 2021
PubMed ID: 34239124
Cryo-EM structure of the periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution
Swanson NA, Lokareddy RK, Li F, Hou CD, Leptihn S, Pavlenok M, Niederweis M, Pumroy RA, Moiseenkova-Bell VY, Cingolani G
Mol Cell. 2021
PubMed ID: 34214465
Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants
Wang L, Zhou T, Zhang Y, Yang ES, Schramm CA, Shi W, Pegu A, Oloniniyi OK, Henry AR, Darko S, Narpala SR, Hatcher C, Martinez DR, Tsybovsky Y, Phung E, Abiona OM, Antia A, Cale EM, Chang LA, Choe M, Corbett KS, Davis RL, DiPiazza AT, Gordon IJ, Helmold Hait S, Hermanus T, Kgagudi P, Laboune F, Leung K, Liu T, Mason RD, Nazzari AF, Novik L, O'Connell S, O'Dell S, Olia AS, Schmidt SD, Stephens T, Stringham CD, Talana CA, Teng IT, Wagner DA, Widge AT, Zhang B, Roederer M, Ledgerwood JE, Ruckwardt TJ, Gaudinski MR, Moore PL, Doria-Rose NA, Baric RS, Graham BS, McDermott AB, Douek DC, Kwong PD, Mascola JR, Sullivan NJ, Misasi J
Science. 2021
PubMed ID: 34210892
Structural insight on assembly-line catalysis in terpene biosynthesis
Faylo JL, van Eeuwen T, Kim HJ, Gorbea Colón JJ, Garcia BA, Murakami K, Christianson DW
Nat Commun. 2021
PubMed ID: 34108468
Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants
Xu J, Xu K, Jung S, Conte A, Lieberman J, Muecksch F, Lorenzi JCC, Park S, Schmidt F, Wang Z, Huang Y, Luo Y, Nair M, Wang P, Schulz JE, Tessarollo L, Bylund T, Chuang GY, Olia AS, Stephens T, Teng IT, Tsybovsky Y, Zhou T, Munster V, Ho DD, Hatziioannou T, Bieniasz PD, Nussenzweig MC, Kwong PD, Casellas R
Nature. 2021
PubMed ID: 34098567
Distinct axial and lateral interactions within homologous filaments dictate the signaling specificity and order of the AIM2-ASC inflammasome
Matyszewski M, Zheng W, Lueck J, Mazanek Z, Mohideen N, Lau AY, Egelman EH, Sohn J
Nat Commun. 2021
PubMed ID: 33980849
DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation
Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
Nature. 2021
PubMed ID: 33731932
Purification and cryoelectron microscopy structure determination of human V-ATPase
Wang L, Chen Z, Wu H, Fu TM
STAR Protoc. 2021
PubMed ID: 33665630
Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes
Steimle S, van Eeuwen T, Ozturk Y, Kim HJ, Braitbard M, Selamoglu N, Garcia BA, Schneidman-Duhovny D, Murakami K, Daldal F
Nat Commun. 2021
PubMedID: 33568648
Structural basis of ribosomal RNA transcription regulation
Shin Y, Qayyum MZ, Pupov D, Esyunina D, Kulbachinskiy A, Murakami KS
Nat Commun. 2021
PubMed ID: 33483500
Structural analysis of cross α-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials
Wang F, Gnewou O, Modlin C, Beltran LC, Xu C, Su Z, Juneja P, Grigoryan G, Egelman EH, Conticello VP
Nat Commun. 2021
PubMed ID: 33462223
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins
Su CC, Lyu M, Morgan CE, Bolla JR, Robinson CV, Yu EW
Nat Methods. 2021
PubMed ID: 33408407
Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative
Pan M, Zheng Q, Yu Y, Ai H, Xie Y, Zeng X, Wang C, Liu L, Zhao M
Nat Commun. 2021
PubMed ID: 33402676
Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases
Kato K, Ahmad S, Zhu Z, Young JM, Mu X, Park S, Malik HS, Hur S
Mol Cell. 2020
PubMed ID: 33373584
Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains
Zhou T, Tsybovsky Y, Gorman J, Rapp M, Cerutti G, Chuang GY, Katsamba PS, Sampson JM, Schön A, Bimela J, Boyington JC, Nazzari A, Olia AS, Shi W, Sastry M, Stephens T, Stuckey J, Teng IT, Wang P, Wang S, Zhang B, Friesner RA, Ho DD, Mascola JR, Shapiro L, Kwong PD
Cell Host Micro. 2020
PubMed ID: 33271067
Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters
Klenotic PA, Moseng MA, Morgan CE, Yu EW
Chem Rev. 2020
PubMed ID: 33211490
Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly
Wang L, Wu D, Robinson CV, Wu H, Fu TM
Mol Cell. 2020
PubMed ID: 33065002
An ER translocon for multi-pass membrane protein biogenesis
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ
Elife. 2020
PubMed ID: 32820719
Structures of filamentous viruses infecting hyperthermophilic archaea explain DNA stabilization in extreme environments
Wang F, Baquero DP, Beltran LC, Su Z, Osinski T, Zheng W, Prangishvili D, Krupovic M, Egelman EH
Proc Natl Acad Sci USA. 2020
PubMed ID: 32759221
Structural mechanism of two gain-of-function cardiac and skeletal RyR mutations at an equivalent site by cryo-EM
Iyer KA, Hu Y, Nayak AR, Kurebayashi N, Murayama T, Samsó M
Sci Adv. 2020
PubMed ID: 32832689
Mechanisms of activation and desensitization of full-length glycine receptor in lipid nanodiscs
Kumar A, Basak S, Rao S, Gicheru Y, Mayer ML, Sansom MSP, Chakrapani S
Nat Commun. 2020
PubMed ID: 32719334
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes
Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS
Science. 2020
PubMed ID: 32703906
The structures of two archaeal type IV pili illuminate evolutionary relationships
Wang F, Baquero DP, Su Z, Beltran LC, Prangishvili D, Krupovic M, Egelman EH
Nat Commun. 2020
PubMed ID: 32647180
Cryo-EM Structure of NPF-bound Human Arp2/3 Complex and Activation Mechanism
Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R
Sci Adv. 2020
PubMed ID: 32548263
Electromechanical coupling in the hyperpolarization-activated K+ channel KAT1
Clark MD, Contreras GF, Shen R, Perozo E
Nature. 2020
PubMed ID: 32461693
Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D
Nat Commun. 2020
PubMed ID: 32358586
Selective PP2A Enhancement through Biased Heterotrimer Stabilization
Leonard D, Huang W, Izadmehr S, O'Connor CM, Wiredja DD, Wang Z, Zaware N, Chen Y, Schlatzer DM, Kiselar J, Vasireddi N, Schüchner S, Perl AL, Galsky MD, Xu W, Brautigan DL, Ogris E, Taylor DJ, Narla G
Cell. 2020
PubMed ID: 32315618
Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
MBio. 2020
PubMed ID: 31964740
Structural basis for COMPASS recognition of an H2B-ubiquitinated nucleosome
Worden EJ, Zhang X, Wolberger C
Elife. 2020
PubMed ID: 31922488
Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS
Reddy B, Bavi N, Lu A, Park Y, Perozo E
Elife. 2019
PubMed ID: 31880537
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway
Butan C, Lara-Tejero M, Li W, Liu J, Galán JE
Proc Natl Acad Sci U S A. 2019
PubMed ID: 31744874
Structure-based characterization of novel TRPV5 inhibitors
Hughes TE, Del Rosario JS, Kapoor A, Yazici AT, Yudin Y, Fluck EC, Filizola M, Rohacs T, Moiseenkova-Bell VY
Elife. 2019
PubMed ID: 31647410
A packing for A-form DNA in an icosahedral virus
Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH
Proc Natl Acad Sci U S A. 2019
PubMedID: 31636205
Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases
Kondo Y, Ognjenovic J, Banerjee S, Karandur D, Merk A, Kullhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J
Science. 2019
PubMed ID: 31604311
Structure of the thermo-sensitive TRP channel TRP1 from the alga Chlamydomonas reinhardtii
McGoldrick LL, Singh AK, Demirkhanyan L, Lin TY, Casner RG, Zakharian E, Sobolevsky AI
Nat Commun. 2019
PubMed ID: 31519888
Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump
Su CC, Morgan CE, Kambakam S, Rajavel M, Scott H, Huang W, Emerson CC, Taylor DJ, Stewart PL, Bonomo RA, Yu EW
MBio. 2019
PubMed ID: 31266873
Ambidextrous helical nanotubes from self-assembly of designed helical hairpin motifs
Hughes SA, Wang F, Wang S, Kreutzberger MAB, Osinski T, Orlova A, Wall JS, Zuo X, Egelman EH, Conticello VP
Proc Natl Acad Sci U S A. 2019
PubMed ID: 31262809
Regulatory switch at the cytoplasmic interface controls TRPV channel gating
Zubcevic L, Borschel WF, Hsu AL, Borgnia MJ, Lee SY
Elife. 2019
PubMed ID: 31070581
Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L
Worden EJ, Hoffman NA, Hicks CW, Wolberger C
Cell. 2019
PubMed ID: 30765112
Cryo-EM structure of the NLRC4CARD filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly
Matyszewski M, Zheng W, Lueck J, Antiochos B, Egelman EH, Sohn J
J Biol Chem. 2018
PubMed ID: 30385506
Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT3A receptor
Basak S, Gicheru Y, Rao S, Sansom MSP, Chakrapani S
Nature. 2018
PubMed ID: 30401837
Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1
Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H
Proc Natl Acad Sci USA. 2018
PubMed ID: 30279182
Structural insights on TRPV5 gating by endogenous modulators
Hughes TET, Pumroy RA, Yazici AT, Kasimova MA, Fluck EC, Huynh KW, Samanta A, Molugu SK, Zhou ZH, Carnevale V, Rohacs T, Moiseenkova-Bell VY
Nat Commun. 2018
PubMed ID: 30305626
Structure and gating mechanism of the transient receptor potential channel TRPV3
Singh AK, McGoldrick LL, Sobolevsky AI
Nat Struct Mol Biol. 2018
PubMed ID: 30127359
Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6
Singh AK, McGoldrick LL, Twomey EC, Sobolevsky AI
Sci Adv. 2018
PubMed ID: 30116787
DNA melting initiates the RAG catalytic pathway
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol. 2018
PubMed ID: 30061602
Cryo-EM structure of the gasdermin A3 membrane pore
Ruan J, Xia S, Liu X, Lieberman J, Wu H
Nature. 2018
PubMed ID: 29695864
Cryo-EM structure of 5-HT3A receptor in its resting conformation
Basak S, Gicheru Y, Samanta A, Molugu SK, Huang W, Fuente M, Hughes T, Taylor DJ, Nieman MT, Moiseenkova-Bell V, Chakrapani S
Nat Commun. 2018
PubMed ID: 29410406